Effects on the survival of rat's abdominal axial skin flap after the combination of VEGF gene therapy and skin flap delay / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the effect of VEGF (vascular endothelial growth factor) gene therapy and skin flap delay on the survival of rat's abdominal axial skin flap.</p><p><b>METHODS</b>In 48 Wistar rats, the model of a abdominal axial skin flap supplied by right superficial epigastric vessel was created. The rats were divided into six groups. The group was treated with subcutaneous injection of pcDNA4-VEGF165, skin flap delay or VEGF injection combined with skin flap delay. 7 days later, the survival rate of the skin flap was measured; specimens were harvested from the skin flap for histological investigation of the microvessels and for immunohistochemical staining to observe the expression of VEGF.</p><p><b>RESULTS</b>Every treated group was significantly higher than blank group in the average survival rate of the skin flap and group V (gene injection when delayed) has the highest one. The average number of the microvessels in group II, III, V, VI was significantly higher than group IV and blank group. Group IV > group V, VI > group II, III > blank group in lumen diameter of the microvessels. Immunohistochemical staining documented more deposition of VEGF DNA in group II, III, V, VI than group IV and blank group.</p><p><b>CONCLUSIONS</b>Both administration of pcDNA4-VEGF165 and skin flap delay can improve the survival of rat's abdominal axial skin flap, but the mechanisms of the effect were different. The combination of the two ways has stronger effect.</p>

The epidemiology character of the 503 residual paralysis of acute flaccid paralysis cases / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Analyze the epidemiology character of the residual paralysis(RP) of acute flaccid paralysis (AFP) in Guangdong during 1994-2007.</p><p><b>METHODS</b>The viruses isolated from the excrement of RP cases were identified and typed in Guangdong from 1994 to 2007. Statistics analysis was performed to reveal the relationship among the immunization history,age,gender and the distribution of the etiology.</p><p><b>RESULTS</b>A total of 503 RP cases were reported. 150 of which were isolated with PV and 59 were isolated with NPEV. From 1994 to 2007, The PV isolating rate ranged from 18.92% to 47.06% and was higher in winter and spring, while the NPEV isolating rate ranged from 4.17% to 25.00%. and was higher in summer and autumn. The PV isolating rate decreased as the age increased,and its isolating rate (61.11%) was highest in "0" year group. The PV isolating rate of the population of < or =2 times OPV was far higher than 3 times. The PV and NPEV isolating rate of the RP cases was higher than without RP.</p><p><b>CONCLUSION</b>The case with RP caused by wild poliovirus wasn't found from 1994 to 2007 in Guangdong, but the relationship of RP case was observed between < or =2 years group and < or =1 time OPV, and NPEVs probably are the potential etiological agent that cause children RP.</p>

Endovascular stent-graft repair for Stanford type A aortic dissection with extra-anatomic bypass / 中华外科杂志

<p><b>UNLABELLED</b>OBJECTIVE To evaluate the effects of endovascular stent-graft repair for Stanford type A aortic dissection combined with extra-anatomic bypass.</p><p><b>METHODS</b>To perform endovascular repair for Stanford type A aortic dissection, we tried to extend the landing zone by extra-anatomic bypass to reconstruct the innominate artery, the left common carotid artery or the left subclavian artery, and then achieved the process immediately or at a secondary stage via either the carotid or the femoral approach.</p><p><b>RESULTS</b>Thirty-four patients with ascending aortic dissection (n=8) and aortic arch dissection (n=26) were treated with this technique. Thirty three patients were successfully done aortic endovascular repair, only one died during the operation. The thirty-day mortality rate was 8.8% (3/34), endoleak incidence rate was 11.8% (4/34) and incidence rate of cerebral infarction was 5.9% (2/34). Twenty-nine patients were followed-up for 6-70 months (mean, 24. 5 months). Complete (n=16) and partial (n=13) thrombosis of the false lumen were showed with CT angiography and/or vascular color Doppler ultrasound scanning.</p><p><b>CONCLUSIONS</b>Endovascular stent-graft repair combined with extra-anatomic bypass can be a novel option for Stanford type A aortic dissection; it is safe, less invasive, and with fewer complications. Nevertheless, indications need further consideration.</p>

Clinical analysis of mesenteric venous thrombosis:a report of 23 cases / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To assess the early clinical diagnosis and treatment of mesenteric venous thrombosis (MVT).</p><p><b>METHODS</b>Clinical data of 23 cases with MVT from January 1994 to December 2003 were analyzed retrospectively.</p><p><b>RESULTS</b>There were 17 males and 6 females, the age ranged from 19 to 74 years old with a mean age of 42 years. Of them, 20 patients presented acute MVT. The main symptoms included abdominal pain and distention, nausea, vomiting, and bloody stool. The detect able rates of transabdominal color Doppler ultrasonography and CT for MVT were 94.1% and 100% respectively. Nine of 11 (81.8%) patients were cured with non-surgical management. Twelve patients underwent surgical treatments including resection of the infarcted bowel and open mesenteric venous thrombectomy with Fogarty catheter via a branch of mesenteric vein. The in-hospital mortality rate was 8.7%, and the postoperative morbidity rate was 33.3%, including ascites in 2 patients and postprandial abdominal pain in other 2 patients. After follow-up from 2 months to nine years, 3 patients had MVT recurrence because of ceasing anti-coagulation treatment and 3 died of myocardial infarction, liver cancer and hepatic cirrhosis.</p><p><b>CONCLUSION</b>Color Doppler ultrasonography and CT scanning are valuable diagnostic methods for MVT, and anticoagulation treatment and operation are effective managements.</p>